Antioxidative Activity of Collagen Peptides Extracted from Cod Bone

Collagen was prepared from cod bone by hot water extraction,and then it was enzymolyzed by neutral protease,protamex,acidic protease and pepsin respectively to obtain collagen peptides.The scavenging capacities to.OH,DPPH.and O2-.free radicals were selected to assay the antioxidant activities of different collagen peptides from cod bone.Results indicated that the scavenging capacities of four kinds of collagen peptide from cod bone to free radicals enhanced with the increase of sample concentration.However,the capacity of scavenging three kinds of free radicals was different for every collagen peptide.They had the strongest scavenging capacity to.OH compared with other two free radicals,and the better scavenging capacity to DPPH.than O2-.when the samples' concentration were 4~10 mg/mL.