Study on Extraction and Characteristics of Deer Skin's Collagen

Collagen is widely used in medical,food and cosmetic fields because of its unique amino acid composition and structure.Deer skin was selected as raw materials to extract collagen through combination with acid hydrolysis and enzymatic hydrolysis,such method could achieve 32.38% extraction rate of collagen.Then it was separated and purified.Content of hydroxyproline in raw materials was determined and content of its collagen was obtained through conversion,which was9.912%.Molecular weight of collagen was measured by SDS-PAGE,which was about 300 thousand,and its free radical scavenging capacity was studied.Within the range of measurement,the free radical scavenging capacity increased as collagen concentrations increased.Collagen had strongly scavenging capacity at high concentration.