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Effect of Dexamathasone on Glomerulus Mesangial Cell Proliferation in Rats

Jian Xiao

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Abstract

Objective To study the effect of dexamethasone (DXM) on the proliferation of rat glomerulus mesangial cells (GMCs). Methods The GMCs lines of the rat were cultured in virto. Both DXM and lipopolysaccharide (LPS) or LPS alone were added into the culture medium (DXM group or LPS group); in the others, neither DXM nor LPS was added and they were used as controls. The GMCs proliferative level was detected by the MTT method at 24 and 48 h of the experiment. Results The GMCs proliferative level in the DXM group of 4 000 ng/well at 48 h [( 0.251 ± 0.056 ) ng/L] was lower than that of the LPS group [( 0.787 ± 0.110 ) ng/L] and the controls [( 0.678 ± 0.101 ) ng/L](P 0.05 ); and the GMCs proliferative inhibition reached a peak at 48 h. Conclusions DXM may inhibit the proliferation of GMCs in rats.

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What this paper is about

Objective To study the effect of dexamethasone (DXM) on the proliferation of rat glomerulus mesangial cells (GMCs). Methods The GMCs lines of the rat were cultured in virto. Both DXM and lipopolysaccharide (LPS) or LPS alone were added into the culture medium (DXM group or LPS group); in the others, neither DXM nor LPS was added and they were used as controls. The GMCs proliferative level was detected by the MTT method at 24 and 48 h of the experiment. Results The GMCs proliferative level in the DXM group of 4 000 ng/well at 48 h [( 0.251 ± 0.056 ) ng/L] was lower than that of the LPS group [( 0.787 ± 0.110 ) ng/L] and the controls [( 0.678 ± 0.101 ) ng/L](P 0.05 ); and the GMCs proliferative inhibition reached a peak at 48 h. Conclusions DXM may inhibit the proliferation of GMCs in rats.

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Available abstract

Objective To study the effect of dexamethasone (DXM) on the proliferation of rat glomerulus mesangial cells (GMCs). Methods The GMCs lines of the rat were cultured in virto. Both DXM and lipopolysaccharide (LPS) or LPS alone were added into the culture medium (DXM group or LPS group); in the others, neither DXM nor LPS was added and they were used as controls. The GMCs proliferative level was detected by the MTT method at 24 and 48 h of the experiment. Results The GMCs proliferative level in the DXM group of 4 000 ng/well at 48 h [( 0.251 ± 0.056 ) ng/L] was lower than that of the LPS group [( 0.787 ± 0.110 ) ng/L] and the controls [( 0.678 ± 0.101 ) ng/L](P 0.05 ); and the GMCs proliferative inhibition reached a peak at 48 h. Conclusions DXM may inhibit the proliferation of GMCs in rats.

Key concepts: Lipopolysaccharide, Dexamethasone, Glomerulus, Endocrinology, Internal medicine, Chemistry, Mesangial cell, Cell growth

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