Determination of Prochloraz Residues in Mushroom with Solid Phase Extraction-High Performance Liquid Chromatography

Determination of prochloraz residues in mushroom by solid phase extraction-high performance liquid chromatography(SPE-HPLC) has been conducted.In the method,acetonitrile and celite545 were added into representative sample,and the mixture was homogenized at ultra high speed.After filtration,the extract was purified by a liquid-liquid partitioning process with NaCl solution and dichloromethane.The dichloromethane phase was evaporated to dryness,and the residue was dissolved with acetonitrile.Then,the organic phase was purified by PSA and C18 SPE tubes.At last,after filtrated with millipore filter,the prochloraz was determined by HPLC.The limit of detection was 0.038 mg/kg,the mean recoveries of prochloraz in the fortified mushroom samples ranged from 82.6% to 100.3%,and the relative standard deviations(RSDs) were in a range of 1.8%~9.2%.The proposed method is simple,rapid,economic and has good linearity.