Experimental Study on Apoptosis of Human Gastric Carcinoma SGC-7901 Cells Induced by Ethanol Extracts of Solanum lyratum

OBJECTIVE: To explore the effect of ethanol extracts of Solanum lyratum on apoptosis of SGC-7901 cells and the mRNA expression of apoptosis associated genes Fas and caspase-3.METHODS:SGC-7901 Cells were cultured in vitro and randomly divided into the contral group,ethanol extracts of S.lyratum treatment groups(2.5 mg·mL-1,5 mg·mL-1,10 mg·mL-1) and cisplatin group.The proliferation inhibitory rate was evaluated by MTT assay.Morphological changes and apoptosis of SGC7901 cells were observed under fluorescence microscope.The mRNA expression of Fas gene and caspase-3 were detected by semi-quantitive RT-PCR method.RESULTS: As compared with control group,the cell proliferation inhibitory rate and apoptosis rate of SGC-7901 cells were increased obviously in S.lyratum treatment groups(P0.01).Obvious morphological change of the SGC-7901 cells represented as the nuclear shrinkage,chromatin condensation and margination;the mRNA expression of Fas gene and caspase-3 were obviously increased as long as concentration of ethanol extract of S.lyratum increased(P0.01或P0.05).CONCLUSION: Ethanol extract of S.lyratum can dose-dependent induced apoptosis and inhibit proliferation,which are associated with regulating the mRNA expression of Fas gene and caspase-3.