Effects of Solanum Lyratum Thumb Extract on Notch1 Gene Expression and Apoptosis in Human Stomach SGC-7901 Cells

Objective:To investigate the effects of Solanum Lyratum Thunb Extract(SLT)on apoptosis and the expression of Notch1,Caspase-9 genes in Human stomach cancer SGC-7901 cells.Methods:Dried whole herbs of Solanum Lyratum Thunb were extracted by boiling distilled water.Human Stomach Cancer SGC-7901 Cells were randomly divided into the control group and SLT-treated groups(12.5mg/mL,25mg/mL,50mg/mL),the growth inhibitory rate was evaluated by MTT assay,cell apoptosis rate was determined by flow cytometry;Expression of Notch1,Caspase-9 mRNA were detected by semi-quantitive RT-PCR,Expression level of Notch1 and Caspase-9 proteins were detected by two-step immunhistochemical staining.Results:Compared with control group,the proliferation inhibitory rate and apoptosis rate were increased obviously(P0.05),the expression of Notch1 mRNA and protein were obvious decreased respectively(P0.05),the expression of Caspase-9 mRNA and protein were obvious increased respectively(P0.05),and display a effect in a dose-dependent manner in the SGC-7901 cells of the SLT-treated groups.Conclusion:SLT can downregulate expression level of Notch1 and induce apoptosis of the Human stomach cancer SGC-7901 cells.