2010•Zhongguo renshougonghuanbing zazhiRequires access
Characterization of Mycobacterium tuberculosis isolates from patients in Gansu by multiple locus variable numbers of tandem repeats.
Chongxiang Tong, Xiuqin Zhao, Jianjun Ma, Zhiguang Liu, Cao WenJiang, Yang Yong-hong, Bing Lü, Yuan Jiang, Wan Kang-lin
Open publisher page 2 citations
Abstract
To analyze the genotype of epidemic Mycobacterium tuberculosis strains by multiple-locus variable-number tandem repeat analysis(MLVA)in Gansu from 2004 to 2006,15 standardized VNTR loci were selected to type the clinical isolated M.tuberculosis strains.Results were analyzed by BioNumerics 4.5 and the dendrogram was summarized from finger printings.Result of MLVA showed that the 228 strains of M.tuberculosis were classified into 4 major genetic groups and 7 main genotypes,which including strains of 13(5.7%),3(1.3%),7(3.1%),1(0.4%),171(75%),31(13.6%)and 2(0.9%),respectively.Furtherly,228 strains were divided into 132 geno-clusters,among which there were 93 strains belonged to unique genotype.Results indicate that the bacterial strains of M.tb in Gansu are rich in genetic polymorphism and MLVA is very useful for typing the genotype of M.tuberculosis strains.Beijing family was the dominant genotype in Gansu.