Effects of DSS on lipid metabolism of Ovariectomized rats induced by Leptin

Objective: To explore possible mechanism of the suppressive effects of 3'-daidzein sulfonate(DSS) on lipid metabolism in ovariectomized(Ovx) rats.Methods: Sixty healthy female SD rats with weight of 160 ~ 220 g were respectively divided into the six groups randomly: sham-operated group(saline),ovariectomized group(Ovx + saline),estradiol valerate group(Ovx + 0.8 mg / kg) and DSS groups(Ovx + 100 /50 /25 μg / kg).Every week,the weight and stem length were measured.All rats were treated for 6 weeks,and serum samples were collected to determine the high density lipoprotein(HDL),low or very low density lipoprotein(LDL/VLDL),triglycerides(TG),or cholesterol(CHO).And the density of leptin or visfatin in serum is detected by radio-immunity.Results: It showed that DSS significantly decreased Lee's index,and increased HDL,but reduced LDL / VLDL,TG,or CHO in serum.These findings indicated that DSS was effective in suppressing the obesity in ovariectomized rats.More specifically,leptin was decreased in serum from the rats by DSS,but increased by estradiol valerate.Conclusion: It is possible that DSS will maintain an efficient level of circulating leptin,thus preventing them in sensitivity to regulate the lipid metabolism and weight gain.It may represent a potential alternative therapy in the treatment of obesity abnormality in ovarian hormone-deficient women.