Effects of Omapatrilat on the Expression of Myocardium MMP-2,MMP-9 and TIMP-1 After Myocardial Infarction in Rats

Objective: To investigate the effect of vasopeptidase inhibitor on ventricular remodelling,cardiac function,expression of matrix metalloproteinase-2(MMP-2),-9(MMP-9),and tissue inhibitors of metalloproteinase-1(TIMP-1) after myocardial infarction in rats.Methods: Male Sprague-Dawley(SD) rats were randomly divided into sham,MI and MI+Omapatrilat(OMA) group.Myocardial infarction was produced by ligation of the left anterior descending coronary artery.After 24 hours of operation,rats in the MI + OMA group were treated with 40 mg/(kg·d) OMA in their drinking water,and rats in the sham and MI groups were treated with placebo.The hemodynamic and ventricular index,as well as the expression of MMP-2 and-9 protein,TIMP-1 protein and collagen volume fraction(CVF) in the noninfarcted zone was determined in the 4th week after operation.Results: The expression of MMP-2,MMP-9 and TIMP-1 protein in the non-infarcted zone was significantly increased in MI group than that in sham group respectively.OMA significantly reduced MMP-2 and MMP-9 protein expression,increased TIMP-1 protein expression,improved ventricular function and ventricular remodelling.CVF in the MI+OMA group decreased than that in the MI group at 4 weeks after operation.Conclusion: The vasopeptidase inhibitor shows favorable effects on left ventricular remodeling after MI in rats and improves cardiac function.These effects may include the downregulation of MMP-2 and MMP-9 expression,and upregulation of TIMP-1 expression in left ventricular.