Effect of omapatrilat on myocardium TNF-α、MMP-2 expression and ventricular remodeling (after) myocardial infarction in rats

OBJECTIVE To investigate the effect of vasopeptidase inhibitor on ventricular remodeling,cardiac function,tumor necrosis factor-α protein and matrix metalloproteinase-2 after myocardial infarction in rats.METHODS Male Sprague-Dawley(SD) rats were randomly divided into three groups: sham,MI and MI + omapatrilat(OMA).Myocardial infarction was produced by ligation of the left anterior descending coronary artery.After 24 hours of operation,rats in the MI + OMA group were treated with 40 mg·kg~(-1)·d~(-1) OMA in their drinking water,and rats in the sham and MI groups were treated with placebo.Determine the hemodynamic and ventricular index as well as the expression of TNF-α protein and MMP2 protein at 6 weeks after operation.RESULTS The expression of TNF-α protein and MMP-2 protein in the non-infarcted zone were significantly increased in MI group than in sham group.OMA significantly reduced TNF-α protein and MMP-2 protein expression,improved ventricular function and ventricular remodeling.CONCLUSION The vasopeptidase inhibitor shows favorable effects on left ventricular remodeling after MI in rats and improves cardiac function.These effects could be relevant to the attenuation of increased MMP-2 and TNF-α in left ventricular.