Study on the Purification and Properties of Superoxide Dismutase from Pomegranate Leaves

The Superoxide dismutase from pomegranate leaves has been isolated and purified by oxganic agent fractionation.optimum technological condition of isolation and purification was studied,and it's property was studied.the results showed that the optimal isolating condition were:the ratio of the pomegranate leaves to the phosphoric acid buffer solution was 1∶2(g/mL),the phosphoric acid buffer solution pH value was 7.6,the extractive time was 1.5 h.the chloroform-ethyl alcohol mix solution amount was 0.25 time of extracts,the acetone amount was 1.0 time of crude enzyme fluid.the enzymatic activity is stable within the temperature 0-60 ℃ and pH 4-9,Through treatment with H_2O_2 or ethanolchloroform,the results indicated the enzyme belongs to Cu·Zn-SOD.This study supplied new enzyme resource for Superoxide dismutase.