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2005•Chinese Journal of Appplied Environmental BiologyRequires access

CONCENTRATION OF PUFAs IN GLYCERIDE BY HYDROLYSIS OF FISH OIL WITH LIPASE

Yi Zheng

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Abstract

Increasing PUFAs (polyunsaturated fatty acids) in glyceride of fish oil by selective hydrolysis with lipase was studied. Through selection of 5 lipases from different sources, the lipase from Aspergillus oryzae showed the best quality. The conditions for hydrolysis of fish oil with this lipase were optimized, including temperature, rotate speed, ratio of oil to water, amount of lipase and additives. Finally, the optimum time for enriching PUFAs in glyceride was confirmed. The results showed under the hydrolysis conditions the content of EPA was increased from 3.0 % to 9.0 %, DHA from 4.3 % to 16.5 %, and DHA + EPA from 7.3 % to 25.5 %. Fig 3, Tab 1, Ref 8

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What this paper is about

Increasing PUFAs (polyunsaturated fatty acids) in glyceride of fish oil by selective hydrolysis with lipase was studied. Through selection of 5 lipases from different sources, the lipase from Aspergillus oryzae showed the best quality. The conditions for hydrolysis of fish oil with this lipase were optimized, including temperature, rotate speed, ratio of oil to water, amount of lipase and additives. Finally, the optimum time for enriching PUFAs in glyceride was confirmed. The results showed under the hydrolysis conditions the content of EPA was increased from 3.0 % to 9.0 %, DHA from 4.3 % to 16.5 %, and DHA + EPA from 7.3 % to 25.5 %. Fig 3, Tab 1, Ref 8

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Available abstract

Increasing PUFAs (polyunsaturated fatty acids) in glyceride of fish oil by selective hydrolysis with lipase was studied. Through selection of 5 lipases from different sources, the lipase from Aspergillus oryzae showed the best quality. The conditions for hydrolysis of fish oil with this lipase were optimized, including temperature, rotate speed, ratio of oil to water, amount of lipase and additives. Finally, the optimum time for enriching PUFAs in glyceride was confirmed. The results showed under the hydrolysis conditions the content of EPA was increased from 3.0 % to 9.0 %, DHA from 4.3 % to 16.5 %, and DHA + EPA from 7.3 % to 25.5 %. Fig 3, Tab 1, Ref 8

Key concepts: Glyceride, Lipase, Polyunsaturated fatty acid, Hydrolysis, Fish oil, Chemistry, Food science, Triacylglycerol lipase

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CONCENTRATION OF PUFAs IN GLYCERIDE BY HYDROLYSIS OF FISH OIL WITH LIPASE — Research Paper | ScholarLens