Development of TaqMan fluorescent quantitative PCR method for detecting classical swine fever virus(CSFV)

A pair of primers and a TaqMan probe were designed according to the CSFV 5′UTR sequences of CSFV taken from GenBank,for performing TaqMan fluorescent quantitative PCR.RNA of classical swine fever virus(CSFV) was used as template,and the reaction conditions of TaqMan fluorescence PCR were optimized based on conventional PCR.The TaqMan fluorescent quantitative PCR method for CSFV was developed,which showed high specificity and high sensitivity to CSFV(detection sensitivity of 5.03×10-2 μg/μL),as revealed by the sensitivity and specificity tests.Therefore,this method can be used for rapid detection of CSFV.