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2004•Wuhan University JournalRequires access

Rapid Quantitative Detection of Classical Swine Fever Virus by Real-time TaqMan Assay

Chuyu Zhang

Open publisher page 2 citations

Abstract

A real-time TaqMan method was developed for detection of classical swine fever virus(CSFV).According to our results, CSFV was detected specifically from all kinds of tissues and blood specimens from pigs experimentally infected with high virulent CSFV,and moderate、low virulent strains of CSFV respectively. It has a sensitivity of 10 copies/μL.By serial dilution of CSFV samples, the TaqMan method was found to 100-fold times more sensitive than RT\|PCR, and it can avoid contamination with non-specific amplicons by post-PCR analysis. Therefore, it was sensitive、specific and rapid for early diagnose of CSFV-related infection.

About this research paper

What this paper is about

A real-time TaqMan method was developed for detection of classical swine fever virus(CSFV).According to our results, CSFV was detected specifically from all kinds of tissues and blood specimens from pigs experimentally infected with high virulent CSFV,and moderate、low virulent strains of CSFV respectively. It has a sensitivity of 10 copies/μL.By serial dilution of CSFV samples, the TaqMan method was found to 100-fold times more sensitive than RT\|PCR, and it can avoid contamination with non-specific amplicons by post-PCR analysis. Therefore, it was sensitive、specific and rapid for early diagnose of CSFV-related infection.

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Available abstract

A real-time TaqMan method was developed for detection of classical swine fever virus(CSFV).According to our results, CSFV was detected specifically from all kinds of tissues and blood specimens from pigs experimentally infected with high virulent CSFV,and moderate、low virulent strains of CSFV respectively. It has a sensitivity of 10 copies/μL.By serial dilution of CSFV samples, the TaqMan method was found to 100-fold times more sensitive than RT\|PCR, and it can avoid contamination with non-specific amplicons by post-PCR analysis. Therefore, it was sensitive、specific and rapid for early diagnose of CSFV-related infection.

Key concepts: Classical swine fever, TaqMan, Virology, Amplicon, Virus, Virulence, Real-time polymerase chain reaction, Biology

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