Effect of KLT combined with DDP on the Proliferation and Apoptosis of SiHa cell

Objective: To investigate the effect of KLT combined with DDP on the proliferation and apoptosis of SiHa cell. Methods: Different concentrations of KLT injection(1, 2, 4, 6, 8 mg/ml)and DDP(1.5, 3, 6, 9, 12 μg/mL)were solo applied to SiHa cells. Cell proliferation was measured by MTT, cell apoptosis was detected by flow cytometry. The appropriate drug concentration(KLT 6 mg/ml,DDP 3 μg/mL)were selected. Then, KLT combined with DDP injection was applied to SiHa cells. Cell proliferation was measured by MTT, cell apoptosis was detected by flow cytometry. Results: ① Cell proliferation were dramatically inhibited by KLT injection(25,50,100,150,200 μg/mL)and DDP(1.5, 3, 6, 9, 12 μg/mL)at 24 h,48 h(P0.05). In addition, Cell proliferation were dramatically inhibited by injected KLT combined with DDP. ②Combination therapy, apoptosis rate and inhibition rate of proliferation in SiHa cells treated by KLT combined with DDP were higher than the monotherapy. Conclusion: DDP, KLT and DDP combined with KLT can inhibit the proliferation, accelerate the apoptosis in SiHa cells. What's more, DDP combined with KLT have synergistic effect on the apoptosis in SiHa cells.