Apoptosis induced by DDP in human SCLC cell line NCI-H446 cells

Background and purpose:Recent studies show that apoptosis induced by chemotherapeutic drugs may be involved in the different steps of nuclear metabolism.Whether small cell lung cancer(SCLC) cells apoptosis induced by cisplatin(DDP) associated with the same molecular mechanism is still under investigation.The purpose of our study was to discover the mechanism of human SCLC cell line H446 cell apoptosis induced by DDP.Methods:Apoptosis induced by DDP in human SCLC cell line H446 was detected by cell morphology,flow cytometry analysis and DNA end labeling,respectively.Results:H446 cells were treated with the concentrations of 0.1, 0.5,1.0,2.0 and 5.0 μg/ml DDP for 48 hr,respectively.Apoptotic index were 1.47±0.03,8.28±0.54,11.02±1.05,12.74±1.08,27.16±1.15,respectively and apoptosis was induced in both a concentration dependent manner and gradually in a time dependent pattern from 12 to 72 hr with a final concentration of 2.0 μg/ml;H446 cells were blocked either at G2 by the final concentration of 0.1,0.5,1.0 and 2.0 μg/ml DDP or at S phase with the final concentration of 5.0 μg/ml DDP.Conclusions:Cell apoptosis induced by DDP may play an important role in the mechanism of DDP antitumor effect.