Expression of Survivin gene in hematological tumour cell lines

Objective To investigate the expression profile of Survivin gene in hematological tumour cell lines. Method The transcriptional levels of Survivin gene were detected by RT-PCR. Results Survivin gene was expressed in 11 hematological tumout cell lines(K562,HL-60,U937,HEL,6T-CEM,NB4,Jurkat,Raji,MUTZ-1,Dami and K562/ADR cell lines), in which the transcriptional levels were significantly higher than those in disease controls. In addition, there existed differential expression levels of Survivin gene in hematological tumour cell lines(NB4MUTZ-1K562RajiHL-606T-CEMU937HELJurkatDami).The transcriptional levels of Survivin gene in K562/ADR cell line increased 1.6-fold compared with the parent K562 cell line. The Survivin expression was not detected in peripheral blood mononuclear cells. All-trans retinoic acid significantly decreased Survivin mRNA expression levels. Conclusion The Survivin gene might be a potential therapeutic target due to the role in anti-apoptosis and drug resistance.