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2011•Unpublished venueRequires access

Research on induction of Callus and Adventitious Buds from F.unibracteata

LI Jun-li

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Abstract

Objictive: Study on the induction of callus and adventitious buds from F.unibracteata. Methods: Explants from the fresh bulb-scale sections of F.unibracteata were in-vitro cultured using Murashige and Skoog (MS) medium supplemented with different ratios concentration of NAA and 6-BA. Results: The fresh bulb-scale sections could be induced to the callus and adventitious buds. The callus induction rate of the explants in MS medium with NAA(1.2 mg/L) and 6-BA(1.8 mg/L) was the highest, reaching 53.3%. The adventitious buds induction rate of the explants in MS medium with NAA(1.2 mg/L) and 6-BA(1.6 mg/L) was the highest, reaching 73.3%. Conclusion: Th is study established the culture methods of callus and adventitious buds from F.unibracteata.

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What this paper is about

Objictive: Study on the induction of callus and adventitious buds from F.unibracteata. Methods: Explants from the fresh bulb-scale sections of F.unibracteata were in-vitro cultured using Murashige and Skoog (MS) medium supplemented with different ratios concentration of NAA and 6-BA. Results: The fresh bulb-scale sections could be induced to the callus and adventitious buds. The callus induction rate of the explants in MS medium with NAA(1.2 mg/L) and 6-BA(1.8 mg/L) was the highest, reaching 53.3%. The adventitious buds induction rate of the explants in MS medium with NAA(1.2 mg/L) and 6-BA(1.6 mg/L) was the highest, reaching 73.3%. Conclusion: Th is study established the culture methods of callus and adventitious buds from F.unibracteata.

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Available abstract

Objictive: Study on the induction of callus and adventitious buds from F.unibracteata. Methods: Explants from the fresh bulb-scale sections of F.unibracteata were in-vitro cultured using Murashige and Skoog (MS) medium supplemented with different ratios concentration of NAA and 6-BA. Results: The fresh bulb-scale sections could be induced to the callus and adventitious buds. The callus induction rate of the explants in MS medium with NAA(1.2 mg/L) and 6-BA(1.8 mg/L) was the highest, reaching 53.3%. The adventitious buds induction rate of the explants in MS medium with NAA(1.2 mg/L) and 6-BA(1.6 mg/L) was the highest, reaching 73.3%. Conclusion: Th is study established the culture methods of callus and adventitious buds from F.unibracteata.

Key concepts: Callus, Explant culture, Botany, Bulb, In vitro, Tissue culture, Biology, Murashige and Skoog medium

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