Construction and Transformation of the Plant Expression Vector Carrying orf224 Gene of Chinese Cabbage

In order to create a new cytoplasmic male sterile line of Chinese cabbage,the recombinant plasmid and expression vector pWR306 from double-enzyme digestion were linked directionally with the segments of CMS7311-orf224 gene to construct the plant expression vector pWR-CMS7311-orf224,which was introduced into Agrobacterium strain EHA105 by means of rapid frozen thaw method.The results of PCR amplification and restriction digestion showed that the fragment of CMS7311-orf224 gene was introduced into pWR306 plasmid and the expression vector pWR-CMS7311-orf224 was transferred into Agrobacterium successfully.By GUS,PCR and RT-PCR preliminary testing,we obtained two 06J28 transgenic lines.