The relationgship between uncoupling protein gene mRNA expression and insulin resistance induced by feeding high-fat.

Objective To explore the relationship between insulin sensitivity and uncoupling protein gene(UCP) mRNA expression in high-fat rat model of insulin resistance. Methods Twenty Wistar rats were randomly divided into to general feed group(n=10) and high-fat feed group(n=10). The model of insulin resistance was made by feeding high-fat. In the experimental study, the change of insulin sensitivity was valued by euglycomic-hyperinsulin clamp and the change of UCP mRNA in liver and muscle was measured by using RT-PCR. Results In the high-fat feed group, the body weight of animal was increased significantly; glucose disposal rate was reduced significantly(GIR, 28.03±8.02 vs 9.89±3.28, P 0.01); and the expression of UCP-2 mRNA in liver and UCP-3 mRNA in muscle decreased remarkably(0.63±0.18 vs 0.43±0.15, P 0.05;1.22±0.24 vs 0.78±0.17, P 0.01). Conclusions The high-fat diet can result in the reduced insulin sensitivity. Its causes may be related with the decreased UCPs gene mRNA expression.