2006•Journal of Clinical UrologyRequires access

Effects of small interfering RNA on CXCR4 expression in LNcap cells

Liu Xiangen

Abstract

Objective:To study the effects of small interfering RNA(siRNA) on the expression of CXCR4 in human prostate cancer cell line Lncap cells.Methods:The recombinant plasmid Psilencer-CXCR4 was transfected into prostate cancer cell line LNcap cells.The cellular CXCR4 expression was detected by RT-PCR.Results:The siRNA could obviously suppress the expression of CXCR4.When transfected with plasmidPsilencer-CXCR4 for 48 h,the levels of the expression of CXCR4 were reduced by 75%(P(0.01)).Conclusions:The siRNA express vector could successfully inhibit the expression of target gene.And it can be potentially useful in the next research of CXCR4.

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Key concepts: LNCaP, Transfection, Small interfering RNA, CXCR4, Medicine, RNA, Cancer research, Prostate cancer