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2009•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access

Effects of small interfering RNA on CXCR4 expression in Jurkat cells

Xiao-Cheng Yin

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Abstract

【Objective】 To study the effects of small interfering RNA(siRNA) on the expression of CXCR4 in Ju-rkat cells.【Methods】 The siRNA was transfected into Acute T-lymphoblastive leukemia of Jurkat cells.The cellu-lar CXCR4 expression was detected by RT-PCR.【Results】 The siRNA could obviously suppression the expression of CXCR4.When transfected with siRNA for 48 h,the levels of the expression of CXCR4 were reduced(P 0.01).【Conclusion】 The siRNA express vector could successfully inhibit the expression of target gene.And it can be potentially useful in the next research of CXCR4.

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What this paper is about

【Objective】 To study the effects of small interfering RNA(siRNA) on the expression of CXCR4 in Ju-rkat cells.【Methods】 The siRNA was transfected into Acute T-lymphoblastive leukemia of Jurkat cells.The cellu-lar CXCR4 expression was detected by RT-PCR.【Results】 The siRNA could obviously suppression the expression of CXCR4.When transfected with siRNA for 48 h,the levels of the expression of CXCR4 were reduced(P 0.01).【Conclusion】 The siRNA express vector could successfully inhibit the expression of target gene.And it can be potentially useful in the next research of CXCR4.

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Available abstract

【Objective】 To study the effects of small interfering RNA(siRNA) on the expression of CXCR4 in Ju-rkat cells.【Methods】 The siRNA was transfected into Acute T-lymphoblastive leukemia of Jurkat cells.The cellu-lar CXCR4 expression was detected by RT-PCR.【Results】 The siRNA could obviously suppression the expression of CXCR4.When transfected with siRNA for 48 h,the levels of the expression of CXCR4 were reduced(P 0.01).【Conclusion】 The siRNA express vector could successfully inhibit the expression of target gene.And it can be potentially useful in the next research of CXCR4.

Key concepts: Jurkat cells, Small interfering RNA, Transfection, Molecular biology, CXCR4, RNA, Vector (molecular biology), Cell biology

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