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Isolation,Identification and Pathogenicity of Newcastle Disease Virus Strains in Chickens of Shandong Province

Xiumei Zhang

Abstract

Six virus strains were isolated from affected chickens in some areas of Shandong province,and characterized as Newcastle disease virus by hemagglutination/hemagglutination_inhibition test,challenge with 6_week specific pathogen free(SPF) chickens and inoculation of embryos after neutralization using particular sera.Mean death time(MDT)of embryos,intracerebral pathogenicity index in 1_day_old chicks(ICP)and intravenous pathogenicity index in 6_week_old chickens(IVPI)were determined respectively for isolates.The results showed that the six strains were highly vilulent and produced different pathogenicity in chickens and embryos.

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Key concepts: Pathogenicity, Biology, Newcastle disease, Virology, Virus, Inoculation, Pathogen, Specific-pathogen-free