2006•Journal of Beijing University of Traditional Chinese MedicineRequires access
Influence of Ganxianyu Formula on collagen metabolism level in rats with immune liver fibrosis
Dianlei Wang
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Abstract
Objective The rat model with immune liver fibrosis was established to study the effect of composite formula-Ganxianyu Formula on the levels of serum laminin(LN),hyaluronic acid(HA), precollagen typeⅢ(PCⅢ)and hydroxyproline(Hyp)in the liver and to investigate the mechanism of Ganxianyu Formula for anti-fibrosis in the liver.Methods The rat model with immune liver fibrosis induced by the human serum albumin was established and then given Ganxianyu Formula orally in low dose(6.1g/kg)and high dose(12.2g/kg)respectively for 8 weeks.Biejiaruangan Tablets or colchicines were taken as the positive control drugs.The levels of serum HA,LN and PCⅢwere detected by radioimmunoassay.The Hyp content in the liver was determined by chemistry method. Meanwhile the pathological changes in the liver tissue were observed.Result In the group with high dose of Ganxianyu Formula the serum levels of HA,LN and PCⅢin rat model with immune liver fibrosis induced by the human serum albumin were decreased significantly,the Hyp content was decreased too,and the histopathological changes in the liver tissue were significantly relieved.Conclusion Ganxianyu Formula has an antagonism on the liver fibrosis through its influence on the collagen metabolism of the liver.