Rapid detection of glufosinate-tolerant transgenic soybean via seed germination method.

We need to choose a repaid and effective method to solve the problem that transgenic offsprings are genetic instable.In this study,the seeds of Dongnong 50 were sowed in MS medium contained different concentrations of glufosinate.Under 5.0 mg·L-1 glufosinate,the germination rate and growth of non-transgenic recipient Dongnong 50 were seriously inhibited and couldn't complete life cycle,while 34.7% of the transgenic soybean could not only germinate and grow up,but also could amplify the right specific segment using CaMV35S and bar primers.We can deduce that the germination method is a rapid and effective method to detect glufosinate-tolerant transgenic soybean.