2010•Dadou kexueRequires access
Comparison on Methods Screening of Transgenic Soybean Resistant to Glufosinate
Hongyu Li
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Abstract
Four screening methods and several screening concentrations were used to screen soybean regeneration plant and the resistance of its progeny as well as to resolve the problem of high false-positive regeneration plants in agrobacterium-mediated transformation of soybean cotyledons after glufosinate screening.After screening the 6802 transgenic plants by spraying the leaf,9 resistant plants were obtained,and the molecular detection of their progeny showed that they were all positive transgenic plants.Therefore,the results indicated that the method that screening the transgenic plant by the leaf spraying was feasible.