The relationship between cytoline,hepatitis B virus and liver fibrosis in the sera of chronic hepatitis B patients

Objective:To observe the effect of cytokines and hepatitis B virus (HBV) on the levels of liver fibrosis in sera of the patients with chronic hepatitis B(CHB).Methods:ELISA and RIA techniques were used to detect the levels transforming growth factor β1(TGF β 1),tumor necrosis factor α(TNF α),interleukin 6(IL 6),interleukin 8(IL 8),hyaline acid(HA),type Ⅲ procollagen(PcⅢ),collagen type Ⅳ(C-Ⅳ),laminin(LN)in 171 patients with chronic hepatitis B and liver cirrhosis,HBV-DNA in serum were determined by quantity PCR.Results:Serum TGF β 1,TNF α,IL 6,IL 8,HA,PCⅢ,C-Ⅳ and LN levels of chronic hepatitis B(CHB) were notable higher than in normal control group(NC),increased successively in middle,moderate and serve degrees of CHB,and were correlated with degree of liver damage positively( P 0 05 or P 0 01).Serum TGF β 1 was correlated with serum levels of HA,PCⅢ,C Ⅳ and LN positively.HBV-DNA(+) in the patients with chronic hepatitis B and liver cirhosis were higher than HBV-DNA(-),and there was significant difference in the moderate and serve chronic hepatitis B patients( P 0 05).Conclusion:The serum of TGF β 1,TNF α,IL 6,IL 8 participates in the mechanism of chronic hepatitis B and in the formation of liver fibrosis,and they were related to active degree replication of HBV.