Effects of Cadmium on DNA Damage in Rat Liver Cells,in Vivo and in Vitro Studies With Single Cell Gel electrophoresis

To study the effects of cadmium chloride on DNA damage of rat liver cells in vivo and in vitro Single cell gel electrophoresis or Comet assay was used At the concentration of 2 185 μmol/L, 4 375 μmol/L, 8 75 μmol/L, 17 50 μmol/L, 35 00 μmol/L, cadmium chloride could induce DNA damage of rat liver cells in vitro, and at the doses of 5 μmol/kg, 10 μmol/kg, 20 μmol/kg, cadmium chloride could induce DNA damage of rat liver cells in vivo respectively The in vivo and in vitro results also showed the obvious dose response relationship between the rates of Comet cells and the doses of cadmium chloride [Conclusion]The research infered that at certain dose, cadmium could induce DNA damage and had toxic effects on rat liver cells