Rotective effects of intestinal RNA on intestinal mucosal barrier in mice after abdominal γ-irradiation

The work was aimed at exploring protective effects of intestinal RNA on intestinal mucosal barrier in mice after abdominal γ-irradiation . The BALB/c male mice were abdominally irradiated with 11.50Gy 60Co γ-ray in 1—3h, and then they were injected intestinal RNA from normal on jejunum. On 1, 3 and 5d after irradiation, the mice were sacrified after anesthesia for determining sIgA in small intestinal mucilage, endotoxin in blood and bacterial metathetic rate, and observing morphological changes of jejunal villus. The result showed that intestinal RNA can de- crease MLN bacterial metathetic rate and the level of endotoxin in blood, and increase sIgA in small intestinal muci- lage (p0.01). In addition, it can improve intestinal mucosal morphology and reduce atrophy and collapse of jejunal villus. In conclusion, Intestinal RNA can improve intestinal mucosal barrier and inhibit intestinal bacterial transloca- tion and absorption of intestinal endotoxin in irradiated mice.