Preliminary of molecule mechanism of rat's infarct brain With VEGF165 gene therapy

Objective To observe the expression of certain molecule in brain of MCAO rats in VEGF 165 gene therapy to provide some molecule evidence for clinical cerebral infarct research.Methods To establish a rat's permanent model of MCAO by nylon suture embolization, VEGF 165 gene was directly injected through skull into the ischemia area,after seven days, the rats were killed,immunohistochemistry was used to show the expression of HSP70 AND VEGF proteins in rats brain.Results There was a significant increase at level of HSP70 and VEGF's expression.Conclusion Ischemic brain tissue could take in and express the VEGF 165 gene, and VEGF 165 gene could induce HSP70 protein.