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The Effect of VEGF on the Expression of Fas and FasL in Ischemic Brain Tissues

Hu Wang

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Abstract

Objective To explore the mechanism of VEGF treating cerebral ischemia. Methods A rat model of middle cerebral artery occlusion (MCAO) was set up by nylon suture embolization. The naked plasmid DNA encoding vascular endothelial growth factor(pUCCAGGS/hVEGF 165 ) was directly injected through skull into the ischemic areas of brain. After seven days the rats were killed. RT-PCR was used to measure the expression of VEGF mRNA in the brain tissues, and immunohistochemistry was appllied to detect the expression of VEGF, Fas and Fas L in the brain tissues. Results Compared with control group, VEGF expression significantly increased in the therapeutic group(P0.01), while Fas and FasL expression obviously decreased in the therapeutic group(P0.01). Conclusion The results indicated that VEGF gene could be introduced into the ischemic brain tissues and express VEGF mRNA and protein. The VEGF protein could protect neuro-cells possibly by inhibting the expression of Fas and FasL.

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What this paper is about

Objective To explore the mechanism of VEGF treating cerebral ischemia. Methods A rat model of middle cerebral artery occlusion (MCAO) was set up by nylon suture embolization. The naked plasmid DNA encoding vascular endothelial growth factor(pUCCAGGS/hVEGF 165 ) was directly injected through skull into the ischemic areas of brain. After seven days the rats were killed. RT-PCR was used to measure the expression of VEGF mRNA in the brain tissues, and immunohistochemistry was appllied to detect the expression of VEGF, Fas and Fas L in the brain tissues. Results Compared with control group, VEGF expression significantly increased in the therapeutic group(P0.01), while Fas and FasL expression obviously decreased in the therapeutic group(P0.01). Conclusion The results indicated that VEGF gene could be introduced into the ischemic brain tissues and express VEGF mRNA and protein. The VEGF protein could protect neuro-cells possibly by inhibting the expression of Fas and FasL.

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Available abstract

Objective To explore the mechanism of VEGF treating cerebral ischemia. Methods A rat model of middle cerebral artery occlusion (MCAO) was set up by nylon suture embolization. The naked plasmid DNA encoding vascular endothelial growth factor(pUCCAGGS/hVEGF 165 ) was directly injected through skull into the ischemic areas of brain. After seven days the rats were killed. RT-PCR was used to measure the expression of VEGF mRNA in the brain tissues, and immunohistochemistry was appllied to detect the expression of VEGF, Fas and Fas L in the brain tissues. Results Compared with control group, VEGF expression significantly increased in the therapeutic group(P0.01), while Fas and FasL expression obviously decreased in the therapeutic group(P0.01). Conclusion The results indicated that VEGF gene could be introduced into the ischemic brain tissues and express VEGF mRNA and protein. The VEGF protein could protect neuro-cells possibly by inhibting the expression of Fas and FasL.

Key concepts: Vascular endothelial growth factor, Fas ligand, Immunohistochemistry, Messenger RNA, Medicine, VEGF receptors, Ischemia, Angiogenesis

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