2010Microbiology, ChinaRequires access

Isolation and Identification of Antagonistic Bacteria Inhibiting Against Sclerotinia sclerotiorum from Sunflower Rhizosphere

Qi Hou

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Abstract

We isolated 640 isolates from sunflower rhizosphere.Eighteen antagonistic bacteria screening have against Sclerotinia sclerotiorum.Isolate XRK5 has obviously and steady antibiosis against the pathogenic fungus,and it implys that XRK5 has good application potentiality.Based on the results of morphological characteristics,physiological and biochemical properties and phylogenetic analysis of 16S rRNA,XRK5 was identified as Lysobacter capsici.The sequence of 16S rRNA obtained in this study has been submitted to GenBank and the accession number is FJ959348.

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What this paper is about

We isolated 640 isolates from sunflower rhizosphere.Eighteen antagonistic bacteria screening have against Sclerotinia sclerotiorum.Isolate XRK5 has obviously and steady antibiosis against the pathogenic fungus,and it implys that XRK5 has good application potentiality.Based on the results of morphological characteristics,physiological and biochemical properties and phylogenetic analysis of 16S rRNA,XRK5 was identified as Lysobacter capsici.The sequence of 16S rRNA obtained in this study has been submitted to GenBank and the accession number is FJ959348.

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Available abstract

We isolated 640 isolates from sunflower rhizosphere.Eighteen antagonistic bacteria screening have against Sclerotinia sclerotiorum.Isolate XRK5 has obviously and steady antibiosis against the pathogenic fungus,and it implys that XRK5 has good application potentiality.Based on the results of morphological characteristics,physiological and biochemical properties and phylogenetic analysis of 16S rRNA,XRK5 was identified as Lysobacter capsici.The sequence of 16S rRNA obtained in this study has been submitted to GenBank and the accession number is FJ959348.

Key concepts: Sclerotinia sclerotiorum, Antibiosis, Biology, Rhizosphere, 16S ribosomal RNA, Sunflower, Sclerotinia, GenBank

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