Isolation of Endophyte from Soybean and Screening of Antagonistic Strains Aganist Sclerotinia sclerotiorum
Wang Chu
Abstract
Wang Chu
Abstract
In order to screen effective antagonistic strains for Sclerotinia sclerotiorum,the endophytes were screened by way of surface sterilization from the different development stages of the soybean root,stem,leaf and seed.The results showed that 113 bacteria and 49 fungus were isolated from different tissues of soybean in its growth stage.42 antagonistic strains against Sclerotinia sclerotiorum were gotten.Among the 42 strains,18strains had been proved that they could effectively inhibit the growth of Sclerotinia sclerotiorum mycelium.
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In order to screen effective antagonistic strains for Sclerotinia sclerotiorum,the endophytes were screened by way of surface sterilization from the different development stages of the soybean root,stem,leaf and seed.The results showed that 113 bacteria and 49 fungus were isolated from different tissues of soybean in its growth stage.42 antagonistic strains against Sclerotinia sclerotiorum were gotten.Among the 42 strains,18strains had been proved that they could effectively inhibit the growth of Sclerotinia sclerotiorum mycelium.
Key concepts: Sclerotinia sclerotiorum, Mycelium, Biology, Sclerotinia, Fungus, Endophyte, Stem rot, Isolation (microbiology)