The comparison of Staphylococcus aureus carrying Panton-Valentine leukocidin gene between children's isolates and adult isolates

Objective To understand the prevalence of Staphylococcus aureus carrying Panton-Valentine leukocidin gene(PVL gene)in isolates from children and adult.Methods The 16SrRNA gene,PVL gene and mecA gene of Staphylococcus aureus from 66 children's isolates and 76 adult isolates were determined by multiplex PCR.After that,the SCCmec genetype and subtype of methicillin-resistant Staphylococcus aureus(MRSA)were determined by the other multiplex PCR.Results Among Staphylococcus aureus strains isolated from 66 children,7 isolates were MRSA(10.6%).Among Staphylococcus aureus strains isolated from 76 adults,39 isolates were MRSA positive(10.6%).The positive rate of MRSA in adult was significantly higher than that in children(χ2 = 26.73,P 0.01).The positive rate of PVL gene in children's isolates and adult isolates were 47.0% and 7.9% respectively.The positive rate of methicillin-sensitive Staphylococcus aureus(MSSA)carrying PVL gene in children's isolates and adult isolates were 49.2% and 5.4% respectively.The differences between children's isolates and adult isolates were all statistically significant(P 0.01).The PVL gene in 2 children isolates was SCCmecⅣ.4 adult MRSA carried PVL gene,one was SCCmecⅠ,one was SCCmecⅡ,and the other two were SCCmecⅢ.Conclusions The rate of Staphylococcus aureus carrying PVL gene in children's isolates is higher than that in adult isolates.The Staphylococcus aureus carrying PVL gene in children's isolates is primarily MSSA.