The detection of Panton-Valentine leukocidin gene of Staphylococcus aureus clinically isolated from children

Objective To understand the infection distribution of Staphylococcus aureus carring Panton-Valentine leukocidin gene(PVL gene) isolated from children. Method The 16S rRNA gene,PVL gene and mecA gene of Staphylococcus aureus were determined by multiplex PCR.The SCCmec gene of MRSA was also determined by multiplex PCR. Result In the total sixty-six strains of Staphylococcus aureus,7 isolates were MRSA(10.6%),59 were MSSA(89.4%);31 isolates were Staphylococcus aureus carring PVL gene;the overall positive rate was 47.0%.Among the 31 isolates,2 isolates were MRSA,29 were MSSA.The two isolates of MRSA carring PVL gene were all SCCmecIV.The thirty-one stains carrying PVL gene were isolated from abscess(21 isolates),blood(7 isolates) and sputum(1 isolates). Conclusion The MSSA from children are the main strains carring PVL gene.Staphylococcus aureus carring PVL gene is associated with suppurative infection and sepsis primarily.