CD58 expression changes induced by experimental high-voltage electrical burns in rat and the therapeutic effects of ulinastatin

Objective To explore the influence of high-voltage electricity on CD58 expression in rats’ blood serum and the therapeutic effects of Ulinastatin (UTI). Methods A total of 180 SD rats were randomly divided into three groups, control group, electrical burn group (burn group) and UTI-treated group (treatment group) and each group contained 60 rats. Voltage regulator and transformer were used to make high-voltage electricity burns (HEB) animal model in the burn and treatment groups. The above equipment- without electricity was used to treat the rats of the control group. Within 2 minutes after electrical burn, 2 mL of normal saline (NS) and UTI (2 × 104 U/kg) were injected intraperitoneally in control and burn group and in treatment group respectively. The collection of cardiac blood and the preparation of the serum were performed at 15 minutes before HEB, 5 minutes later, 1 hour later, 2 hours later, 4 hours later and 8 hours later (10 rats were selected randomly in above time\|point). The concentration of CD58 was assayed with ELISA. And the experimental data were analyzed statistically with variance analysis and LSD test. Results(1) The comparison of CD58 expression between the control and burn groups: the expression in burn group was increased compared with that in control group (F=882.901, P0.01), the increased CD58 expression in burn group was in a time\|dependent manner and the expression at 5 minutes to 8 hours after the burn was higher than that before the burn (F=81.459, P0.01). And there was an increased trend for expression of CD58. (2) The comparison of CD58 expression between the burn and treatment groups: the expression in treatment group was reduced compared with that in burn group (F=219.597, P0.01). The change of CD58 expression in treatment group was linked with time duration and the expression at 5 minutes to 8 hours after the burn was higher than that before the burn (F=155.774, P0.01). ConclusionsCD58 expression was up\|regulated in blood serum of rats after HEB and its expression was reduced after UTI treatment.