Changes of leukocyte adhesion and soluble intercellular adhesion molecule 1 expression induced by transcranial high-voltage electrical burns in rat and the therapeutic effects of Ulinastatin

Objective To investigate the influence of transcranial high-voltage electricity on leukocyte adhesion and soluble intercellular adhesion molecule 1 ( sICAM-1) expression in rats'blood serum and the therapeutic effects of Ulinastatin ( UTI) . Methods One hundred and eighty SD rats were randomly divided into three groups,control group,electrical burn group ( burn group ) and UTI-treated group ( treatment group) and each group contained 60 rats. Voltage regulator and experimental transformer were used to make high-voltage electricity burns ( HEB) animal model. The above equipment without electricity was used to treat the rats of the control group. 2 mL of normal saline ( NS) were intraperitoneal injected into the rats of control or burn group. 2 mL of UTI ( 2 × 10 4 U / kg) were intraperitoneal injected into the rats of treatment group. Ten rats in each group were selected randomly at 15 minutes before HEB,5 minutes later, 1 hour later,2 hours later,4 hours later and 8 hours later. Amount of adhesional leukocyte in the mesenteric venule was detected using the BVPM. sICAM-1 concentration in the serum of the cardiac blood was assayed with ELISA. And the experimental data were analyzed statistically with variance analysis and LSD test. Results ( 1) sICAM-1 expression in burn group was increased compared with that in control group and the change was significant statistically ( F = 536. 550,P 0. 01) . sICAM-1 expression at 5 minutes to 8 hours after the burn was higher than that before the burn and the increase was significant statistically ( F = 70. 371, P 0. 01) . sICAM-1 expression in treatment group was reduced compared with that in burn group and the reduction was significant statistically ( F = 14. 784,P 0. 01) . sICAM-1 expression of treatment group at 5 minutes to 8 hours after the burn was higher than that before the burn,and the change was significant statistically ( F = 126. 391,P 0. 01) . ( 2) Amount of adhesional leukocyte was increased compared with that in control group,and the increase was significant statistically ( F = 1309. 845,P 0. 01) . The amount in burn group at 5 minutes to 8 hours after the burn was higher than that before the burn and the change was significant statistically ( F = 73. 485,P 0. 01 ) . Amount of adhesional leukocyte in treatment group was decreased compared with that in burn group and the reduction was significant statistically ( F = 447. 666,P 0. 01) . The amount in treatment group at 5 minutes to 8 hours after the burn was higher than that before the burn and change was significant statistically ( F = 82. 933,P 0. 01 ) . Conclusions Transcranial HEB up-regulated sICAM-1 expression in blood serum of rats and amount of adhesional leukocyte in the mesenteric venule. And UTI reduced the sICAM-1 expression and the amount of adhesional leukocyte after transcranial HEB.