Pepsin Hydrolysis of Casein for the Preparation of ACE Inhibitory Peptides

The optimal pepsin hydrolysis of casein for preparing ACE inhibitory peptides was explored by single factor and orthogonal array design methods.The optimal hydrolysis parameters were determined to be: 37 ℃,pH 3.0,enzyme-tosubstrate ratio 1:100,and substrate concentration 7%.The optimized pepsin hydrolysis yielded a product presenting 92.5% ACE inhibition ratio and 0.24 mg/mL IC50.The correlation between hydrolysis degree of casein and ACE inhibition ratio of its hydrolysates was complicated.