Optimization of Enzymatic Hydrolysis of Porcine Plasma Protein Powder for Production of ACE-inhibitory Peptides

In this study,pepsin,trypsin,alcalase and neutrase were compared for their effectiveness in hydrolyzing porcine plasma protein powder.Pepsin was found to be the appropriate enzyme to produce ACE-inhibitory peptides.In order to maximize ACE inhibitory rate,the hydrolysis of porcine plasma protein powder by pepsin was optimized by single factor and orthogonal array design methods.Substrate concentration was identified as the most important factor that affects ACE inhibitory rate,followed by hydrolysis time,pH and enzyme/substrate ratio.The effects of substrate concentration and hydrolysis time on ACE inhibitory rate were statistically significant(P0.05),while neither pH nor enzyme/substrate ratio significantly(P0.05) affected ACE inhibitory rate.pH of 2.3,hydrolysis time of 1.5 h,substrate concentration of 1 g/100 mL and enzyme/substrate ratio of 1:6 were found optimum.