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2011•Zhongguo zhongyiyao xinxi zazhiRequires access

Content Determination of Paeoniflorin in Paishilidan Tablets by HPLC

Chen Zhi-cheng

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Abstract

Objective To establish a method for determination of paeoniflorin in Paishilidan tablets by HPLC.Method Hypersil ODS-3 C18 column(4.6 mm×150 mm,5 μm) was used,acetonitrile-0.1% phosphoric acid(18∶82) was the mobile phase,detection wavelength was at 230 nm,and the flow rate was 1.0 mL/min.Result The linear range of paeoniflorin was 0.06～0.36 μg,r=0.999 9,and the average recovery was 99.77% with RSD of 1.35%(n=6).Conclusion The method was simple,quick and accurate,and available for quality control of the preparation.

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What this paper is about

Objective To establish a method for determination of paeoniflorin in Paishilidan tablets by HPLC.Method Hypersil ODS-3 C18 column(4.6 mm×150 mm,5 μm) was used,acetonitrile-0.1% phosphoric acid(18∶82) was the mobile phase,detection wavelength was at 230 nm,and the flow rate was 1.0 mL/min.Result The linear range of paeoniflorin was 0.06～0.36 μg,r=0.999 9,and the average recovery was 99.77% with RSD of 1.35%(n=6).Conclusion The method was simple,quick and accurate,and available for quality control of the preparation.

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Available abstract

Objective To establish a method for determination of paeoniflorin in Paishilidan tablets by HPLC.Method Hypersil ODS-3 C18 column(4.6 mm×150 mm,5 μm) was used,acetonitrile-0.1% phosphoric acid(18∶82) was the mobile phase,detection wavelength was at 230 nm,and the flow rate was 1.0 mL/min.Result The linear range of paeoniflorin was 0.06～0.36 μg,r=0.999 9,and the average recovery was 99.77% with RSD of 1.35%(n=6).Conclusion The method was simple,quick and accurate,and available for quality control of the preparation.

Key concepts: Paeoniflorin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Content determination, Chemistry, Linear range, Linear relationship

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