2009•PubMedRequires access

[Identification and genotyping of oncogenic type of human papillomavirus in paraffin-embedded cervical cancer samples in Guangzhou].

Li Zeng, Shou-yi Yu, Sui-qun Gou, Jin-ping Yun, Jun Zhang

Abstract

OBJECTIVE: To investigate human papillomavirus (HPV) infection and genotyping in patients with cervical cancer in Guangzhou in the last 3 decades. METHODS: HPV L1 gene fragment in paraffin-embedded cervical cancer samples was amplified by HPV-specific PCR with consensus primers, and typing of HPV strains was performed on the basis of sequence analysis of the PCR products. RESULTS: The positivity rates of HPV DNA was 26.2% in the 99 cases of cervical cancer. Five HPV genotypes were identified including HPV16, 18, 33, 52 and 58. CONCLUSION: HPV16, 58 and 33 are the most common genotypes of HPV, accounting for over 88.4% in the total infected cases, suggesting that the HPV genotypes closely related to cervical cancer is more centralized in Guangzhou.

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Key concepts: Genotyping, Genotype, Cervical cancer, Human papillomavirus, Typing, Virology, Polymerase chain reaction, HPV infection