Studies on the pollen storage and viability of 3 oil-tea species

Three oil-tea species including: Camellia oleifera,C.vietnamenisis,C.semiserrata were selected for the studies on the best conditions of pollen storage,suitable culture medium,culture temperature for pollen germination,and the effects of different conditions and different time for pollen storage on pollen viability.The results were summarized as follows: the difference in oil-tea pollen viability under different storage conditions was significant,the longer the storage time,the lower the pollen viability.The pollen viability changed with the storage conditions and time in a different way.The pollen viability in the frozen(-20 ℃) conditions decreased slowest,which kept a high level of viability after three month.The pollen viability under natural conditions decreased rapidly fast,which reached 0 after 10 days.The most suitable germination temperature for pollen was 20 ℃,and the most suitable culture medium was 0.01% HBO3+0.03% Ca(NO3)2·4H2O+0.02% MgSO4·7H2O+0.01% KNO3+15% sucrose+1% agar.