Effect of Macrophage Migration Inhibitory Factor on Proliferation and Apoptosis in Human Gastric Cancer Cell Line BGC-823

Objective To observe the effect of macrophage migration inhibitory factor of hyaluronic acid on proliferation and apoptosis in human gastric cancer cell line BGC-823 in vitro. Methods MTT assay was used to determine the effect on the inhibition of the growth of BGC-823 cell by the drugs.Flow cytometry and AO-EB fluorescent staining were used to determine the effect on the apoptosis of BGC-823 cells by the drugs.Flow cytometry was used to assay the effect on the cell cycle of BGC-823 cells by the drugs. Results The result of MTT assay showed that the growth of BGC-823 cells was inhibited by HA inhibition to MIF with concentration from 100 μg/mL to 400 μg/mL,and the effect increased in a concentration-and time-dependent manner.The results of apoptosis on the cell cycle of BGC-823 cells determined by AO-EB fluorescent stainining and flow cytometry were coincident: compared with the negative control group,apoptosis of HA group was significantly increased,and the rates of apoptosis and rupture of membrane were increased with higher concentration.The result of flow cytometry showed that HA could block off BGC-823 cells in stage of G0/G1,and the role was significantly increased with higher concentration compared with the negative control group. Conclusions MIF can promote the growth and inhibit the apoptosis of human gastric cancer cells BGC-823.