The Inhibit Effect of the Cyclooxygenase-2 Inhibitor Nimesulide on Human Bladder Cancer Cell Line T24 in Vitro

Objective To investigate the growth effects of the selective cyclooxygenase-2 inhibitor Nimesulide on human bladder cancer cell line T24 in vitro, and to clarify its probable mechanism.Methods The T24 cell line was culture in vitro, and exposed to a range of concentrations of Nimesulide at different time. The effect of COX-2 inhibitor Nimesulide on the proliferation and apoptosis of human bladder cancer cell line T24 in vitro were observed by using cell morphology , MTT assay , flow cytometry, fluorescent microscopy.Results Significant cytotoxicity of cyclooxygenase-2 inhibitor Nimesulide on human bladder cancer cell line T24 were observed by cell morphology. MTT assay and flow cytometry show that cyclooxygenase-2 inhibitor Nimesulide significantly inhibited cell proliferation of T24 cell line in vitro in a dose- and time-dependent manner .Cell-cycle analysis by flow cytometry suggested that Nimesulide induced a decrease in S and G2/M phase population and a significant increase in G0/G1 population compared with untreated T24 cells. And a typical subdiploid peak before Phase G0/G1 was detected by flow cytometry. The rates of apoptosis were correlated with the concentration of Nimesulide and time of action. A typical apoptosis morphology were also observed by fluorescent microscopy.Conclusion The selective cyclooxygenase-2 inhibitor Nimesulide has significant inhibit effects on the growth of T24 cell, which is closely related to block cell cycle and apoptosis-inducing.