Cyclooxygenase Inhibitor Suppressed COX-2 Expression and Induced Apoptosis in Human Bladder Cancer Cell Line T24

Objective To study effects of nimesulide in inducing apoptosis and suppressing expression of cyclooxygenase-2(COX-2) in human bladder cancer cell line T24. Methods Human bladder cancer cell line T24 cells were studied and human gastric cancer cell line SGC-7901 was served as positive control cell line.Cells were cultured for 24 hours with nimesulide at different concentrations.Apoptosis was detected by DNA fragmentation electrophoresis, while COX-2 by Western blot. Image Tool software was used to analyze Western blot image. Results With increase of nimesulide concentrations, cell apoptosis increased.COX-2 expression could be obviously suppressed by COX-2 specific inhibitor nimesulide. The concentration of maximum suppressing effect was 100 μmol/L. Software analysis revealed that with the increase nimesulide concentrations, and the expression of COX-2 obviously decreased.Difference was significant(P 0.01).Conclusion Nimesulide could effectively induced apoptosis in T24 cell line, which may be related to the expression inhibition of COX-2.