Effect of 15d-PGJ_2 on proliferation and apoptosis of HSC-T6

Aim To evaluate the effect of 15d-PGJ2 on up-regulated expression of PPARγ and inducing HSC apoptosis and inhibiting HSC proliferation. Methods The rat liver stellate cell line (HSC-T6) was cultured in DMEM, and treated with PPARγ agonist 15d-PGJ2 of different concentrations. The expression of PPARγ mRNA was detected by RT-PCR. The protein expression of NF-κB was examined by Western blot. The cell proliferation rate of HSC-T6 was determined by MTT colorimetric assay. The cell cycle and apoptosis ratio were measured using flow cyto -metry analysis. Results The proliferation rate of the rat liver stellate cell line (HSC-T6) was significantly inhibited by 15d-PGJ2 (vs controls, P0.05). Especially in the concerntration of 2 μmol·L-1 group the proliferation rate was markly decreased, Western blot showed that protein expression of NF-κB decreased in HSC treated with 15d-PGJ2. In addition, acridine orange fluorescence vital staining suggested the apoptotic rate was significantly increased in HSC treated with 15d-PGJ2. Flow cytometry suggested HSC treated with 15d-PGJ2 was blocked in G1 phase. Conclusion 15d-PGJ2 can inhibit the activity of NF-κB so to cut down proliferation of HSC and induce HSC apoptosis.