The effect of H168 on hepatic stellate cell apoptosis and its possible mechanism

Aim To observe the effect of H168 on HSC apoptosis and its possible mechanism.Methods LDH kit was used to detect the effect of H168 on LDH activity;MTT was used to detect the effect of drug on HSC-T6 proliferation;apoptosis was measured by using flow cytometry(FCM) analysis with Annexin-Ⅴ/PI dual staining for apoptotic cell ratio;Western blot was used to detect the protein expression of Bcl-2、Bax and Caspase-3.Results No obvious cytotoxic effect was found when the final concentration of H168 was less than 150 μmol·L-1;MTT found that H168 could inhibit HSC-T6 proliferation at the concentration of 10 μmol·L-1 and 25 μmol·L-1;FCM detection found H168 promoted apoptosis of HSC in a dose-dependent manner;Western blot results showed that H168 could promote HSC-T6 apoptosis by promoting Bax/Bcl-2 and Caspase-3 protein expression.Conclusion H168 has the function of promoting the apoptosis of HSC-T6 by promoting Bax/Bcl-2 and Caspase-3 protein expression.