Study on tissue specific function of mouse albumin promoter

Green fluorescent protein(GFP)gene was used as a reporter gene to study the tissue-specific function of mouse albumin promoter in different deriving cells.Mouse albumin promoter(-215bp to+24bp)was fused to the5'-end of GFP gene in vector pEGFP-C1.The identified recombinant vector pEGFP-C1-P and its control pEGFP-C1were transfected into Hepa1-6(mouse hepatoma cell line),HepG2(human hepatoma cell line),CHO(Chinese ham-ster ovary cell line)and PLA801(human lung cancer cell line),respectively.Albumin promoter could drive GFP transient and stable expression in Hepa1-6and HepG2.Level of GFP transient expression driven by albumin pro-moter is one-fourth of that driven by CMV promoter in Hepa1-6while level of GFP stable expression drived by albumin promoter increased to2fold of that of CMV promoter after2weeks screening with G418.Albumin promot-er could not express GFP in CHO and PLA801.But control CMV promoter expressed GFP very well in these two cell lines.Our results indicated that-215bp to+24bp of albumin gene had the function of liver tissue-specific ex-pression.