Mouse albumin enhancer lost its enhancement function in hepatoma cell line

Objective:To identify the enhancement function of mouse albumin enhancer in the transcription activity of albumin promoter in hepatoma cell lines. Methods:Green fluorescent protein (GFP) gene was used as a reporter gene to study the enhancement function of mouse albumin enhancer in the cells derived from the different tissues. Mouse albumin enhancer fragments of -10.21?kb to -8.43?kb and albumin promoter were fused with the 5′-end of GFP gene in vector pEGFP-C1. The identified recombinant vector and its control vector only containing albumin promoter were transfected into Hepa 1-6 (mouse hepatoma cell line), HepG2 (human hepatoma cell line), CHO (Chinese hamster ovary cell line) and PLA801(human lung cancer cell line), respectively. Results:Albumin promoter could drive GFP transient and stable expression in Hepa 1-6 and HepG2, but could not drive GFP stable expression not in CHO and PLA801. These enhancer fragments could not enhance the transcription of albumin promoter when transiently transfected in Hepa 1-6 and stably transfected in HepG2. On the contrary, these enhancer fragments suppressed the transcription activity of mouse albumin promoter. Albumin promoter can not transcribe the interest gene in non-liver derived cell lines. Conclusion:It is better to use only albumin promoter to express a gene in hepatoma cell lines than to use albumin promoter and its enhancer.