Experimental study of SD rats bone marrow stromal cells induced into osteoblasts in vitro

Objective To study the osteogenic methods and cultural conditions of the SD rats bone marrow stromal cells(BMSCs) induced into osteoblasts in vitro.Methods After obtaining abacterial bone marrow from SD rats femur,the BMSCs were separated and purified by using of whole bone marrow adherent method.Then they were divided into two groups: the experimental group and the control group.In the experimental group,the passage cells of the third generation were cultured in the osteogenic induction medium including dexamethasone,β-sodium glycerophosphate and vitamin C.The growth curve of cultural cells was drawn by MTT colorimetric method.The alkaline phosphatase(ALP) staining of cultural cells was conducted by BCIP/NBT.Calcium tubercle was stained with alizarin bordeaux.The two groups were compared.Results Compared with the control group,the growth velocity of BMSCs cultured in osteogenic induction medium in the experimental group was obviously lower than that in the control group,and BMSCs developed into osteoblasts.The ALP positive rate in the experimental group was 88.34%±8.65%,and the control group was 28.14%±5.38%(P0.01).On the 13th day,the positive orange-red calcium nodules in the experimental group were found,while the control group was almost colorless.Conclusion BMSCs of SD rats have the potential of being induced into the osteoblasts when cultured in vitro.